High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

Antoine J. Ribieras; Yulexi Y. Ortiz; Sophie Shrestha; C. Theodore Huerta; Hongwei Shao; Maria E. Boulina; Roberto I. Vazquez-Padron; Zhao-Jun Liu; Omaida C. Velazquez

doi:10.3791/63284

Journal

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Medicine

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High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

JoVE Journal
Medicine

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JoVE Journal Medicine

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

3,448 Views

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08:16 min

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March 16, 2022

DOI:

10.3791/63284-v

DOI:

10.3791/63284-v

•

08:16 min

March 16, 2022

•

3440 Views

Antoine J. Ribieras¹, Yulexi Y. Ortiz^1,2, Sophie Shrestha³, C. Theodore Huerta¹, Hongwei Shao^1,2, Maria E. Boulina⁴, Roberto I. Vazquez-Padron^1,2, Zhao-Jun Liu^1,2, Omaida C. Velazquez^1,2

¹Dewitt Daughtry Family Department of Surgery,University of Miami Miller School of Medicine ²Vascular Biology Institute,University of Miami Miller School of Medicine ³University of Miami ⁴Analytical Imaging Core Facility,University of Miami

Summary

The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.