The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.
Ribieras, A. J., Ortiz, Y. Y., Shrestha, S., Huerta, C. T., Shao, H., Boulina, M. E., Vazquez-Padron, R. I., Liu, Z., Velazquez, O. C. High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model. J. Vis. Exp. (181), e63284, doi:10.3791/63284 (2022).