-1::1
Simple Hit Counter
Skip to content

Products

Solutions

×
×
Sign In

EN

EN - EnglishCN - 简体中文DE - DeutschES - EspañolKR - 한국어IT - ItalianoFR - FrançaisPT - Português do BrasilPL - PolskiHE - עִבְרִיתRU - РусскийJA - 日本語TR - TürkçeAR - العربية
Sign In Start Free Trial

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
View All
JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

Biological Techniques
Biology
Cancer Research
Immunology
Neuroscience
Microbiology
JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduate courses

Analytical Chemistry
Anatomy and Physiology
Biology
Calculus
Cell Biology
Chemistry
Civil Engineering
Electrical Engineering
View All
JoVE Science Education

Visual demonstrations of key scientific experiments

Advanced Biology
Basic Biology
Chemistry
View All
JoVE Lab Manual

Videos of experiments for undergraduate lab courses

Biology
Chemistry

BUSINESS

JoVE Business

Video textbooks for business education

Accounting
Finance
Macroeconomics
Marketing
Microeconomics

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Authors

Teaching Faculty

Librarians

K12 Schools

Biopharma

Products

RESEARCH

JoVE Journal

Peer reviewed scientific video journal

JoVE Encyclopedia of Experiments

Video encyclopedia of advanced research methods

JoVE Visualize

Visualizing science through experiment videos

EDUCATION

JoVE Core

Video textbooks for undergraduates

JoVE Science Education

Visual demonstrations of key scientific experiments

JoVE Lab Manual

Videos of experiments for undergraduate lab courses

BUSINESS

JoVE Business

Video textbooks for business education

OTHERS

JoVE Quiz

Interactive video based quizzes for formative assessments

Solutions

Authors
Teaching Faculty
Librarians
K12 Schools
Biopharma

Language

English

EN

English

CN

简体中文

DE

Deutsch

ES

Español

KR

한국어

IT

Italiano

FR

Français

PT

Português do Brasil

PL

Polski

HE

עִבְרִית

RU

Русский

JA

日本語

TR

Türkçe

AR

العربية

    Menu

    JoVE Journal

    Behavior

    Biochemistry

    Bioengineering

    Biology

    Cancer Research

    Chemistry

    Developmental Biology

    Engineering

    Environment

    Genetics

    Immunology and Infection

    Medicine

    Neuroscience

    Menu

    JoVE Encyclopedia of Experiments

    Biological Techniques

    Biology

    Cancer Research

    Immunology

    Neuroscience

    Microbiology

    Menu

    JoVE Core

    Analytical Chemistry

    Anatomy and Physiology

    Biology

    Calculus

    Cell Biology

    Chemistry

    Civil Engineering

    Electrical Engineering

    Introduction to Psychology

    Mechanical Engineering

    Medical-Surgical Nursing

    View All

    Menu

    JoVE Science Education

    Advanced Biology

    Basic Biology

    Chemistry

    Clinical Skills

    Engineering

    Environmental Sciences

    Physics

    Psychology

    View All

    Menu

    JoVE Lab Manual

    Biology

    Chemistry

    Menu

    JoVE Business

    Accounting

    Finance

    Macroeconomics

    Marketing

    Microeconomics

Start Free Trial
Loading...
Home
JoVE Journal
Medicine
High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia
High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

Full Text
5,260 Views
05:32 min
January 19, 2022

DOI: 10.3791/63292-v

Rüdiger Schultz1,2, Tiina Peromaa3, Heikki Lukkarinen2, Varpu Elenius2

1Allergy Centre,Tampere University Hospital, 2Department of Pediatrics,Turku University Hospital, 3Turku Bioscience Center,University of Turku and Åbo Akademi University

Overview

High-speed video microscopy analysis is a reliable and cost-effective tool for diagnosing primary ciliary dyskinesia. It is easy to perform and applicable across all age groups, making it essential for centers treating severe lung diseases.

Key Study Components

Area of Science

  • Neuroscience
  • Respiratory Medicine
  • Diagnostic Techniques

Background

  • Primary ciliary dyskinesia is a condition affecting ciliary function.
  • High-speed video microscopy can reveal both primary and secondary ciliary function.
  • This technique is particularly useful for diagnosing respiratory issues related to ciliary dysfunction.
  • It is important for clinicians to have access to reliable diagnostic tools.

Purpose of Study

  • To evaluate the effectiveness of high-speed video microscopy in diagnosing primary ciliary dyskinesia.
  • To assess the applicability of this technique across different patient age groups.
  • To highlight the importance of this diagnostic tool in clinical settings.

Methods Used

  • High-speed video microscopy analysis.
  • Evaluation of ciliary motion and function.
  • Comparison of results across various patient demographics.
  • Assessment of secondary ciliary function in relation to infections.

Main Results

  • High-speed video microscopy is effective for diagnosing primary ciliary dyskinesia.
  • The technique is reliable when performed by experienced personnel.
  • It provides insights into secondary ciliary function.
  • Applicable to patients of all ages, enhancing its utility in clinical practice.

Conclusions

  • High-speed video microscopy should be a standard diagnostic tool in respiratory medicine.
  • Its ease of use and reliability make it suitable for first-line diagnostics.
  • Widespread availability can improve outcomes for patients with severe lung diseases.

Frequently Asked Questions

What is primary ciliary dyskinesia?
Primary ciliary dyskinesia is a genetic disorder that affects the movement of cilia, leading to respiratory issues.
How does high-speed video microscopy work?
It captures rapid movements of cilia, allowing for detailed analysis of their function.
Is this technique suitable for all age groups?
Yes, high-speed video microscopy is applicable for patients of all ages.
What are secondary ciliary functions?
Secondary ciliary functions can be affected by infections, impacting overall respiratory health.
Why is this technique important?
It provides a reliable method for diagnosing conditions that affect ciliary function, crucial for effective treatment.
Can this method be used in all clinical settings?
Yes, it should be available in every center involved in the treatment of severe lung diseases.

High-speed video microscopy analysis is a relatively easy-to-perform, fast, cost-effective, and, in experienced hands, a considerably reliable tool for first-line diagnostics of primary ciliary dyskinesia, which should be available in every center involved in diagnostics and the treatment of severe lung diseases.

High speed Video microscopy analysis is a reliable tool for first line diagnostic of primary ciliary dyskinesia. This technique is relative easy to perform, fast, cost effective, and in experienced hands else a very reliable tool. And it is applicable for patients of all age groups.

Video microscopy also reveals secondary ciliary function. Like, for instance, due to bacterial or viral infections. But it has been mainly used to diagnose primary ciliary dyskinesia.

View the full transcript and gain access to thousands of scientific videos

View the full transcript and gain access to thousands of scientific videos

Sign In Start Free Trial

Explore More Videos

High-speed Video MicroscopyPrimary Ciliary DyskinesiaDiagnostic ToolRespiratory Epithelial CellsIntracellular ProcessesBiological FunctionsSpecimen CollectionMicrocentrifuge TubeVideo AnalysisCell ClustersImmunohistochemistryCilia MovementsROI (region Of Interest)Laboratory TechnicianOil Immersion Lens

Related Videos

Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia

08:25

Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia

Related Videos

21.7K Views

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo

08:00

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo

Related Videos

12.7K Views

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Related Videos

8.8K Views

In vivo Evaluation of Mucociliary Clearance in Mice

06:35

In vivo Evaluation of Mucociliary Clearance in Mice

Related Videos

4.8K Views

Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic

09:03

Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic

Related Videos

5.7K Views

Artificial Intelligence Approaches to Assessing Primary Cilia

08:58

Artificial Intelligence Approaches to Assessing Primary Cilia

Related Videos

4.3K Views

Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency

11:13

Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency

Related Videos

4.9K Views

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

07:40

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

Related Videos

1.4K Views

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

09:53

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

Related Videos

1K Views

FISH for Pre-implantation Genetic Diagnosis

07:34

FISH for Pre-implantation Genetic Diagnosis

Related Videos

38K Views

JoVE logo
Contact Us Recommend to Library
Research
  • JoVE Journal
  • JoVE Encyclopedia of Experiments
  • JoVE Visualize
Business
  • JoVE Business
Education
  • JoVE Core
  • JoVE Science Education
  • JoVE Lab Manual
  • JoVE Quizzes
Solutions
  • Authors
  • Teaching Faculty
  • Librarians
  • K12 Schools
  • Biopharma
About JoVE
  • Overview
  • Leadership
Others
  • JoVE Newsletters
  • JoVE Help Center
  • Blogs
  • JoVE Newsroom
  • Site Maps
Contact Us Recommend to Library
JoVE logo

Copyright © 2026 MyJoVE Corporation. All rights reserved

Privacy Terms of Use Policies
WeChat QR code