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JoVE Journal Developmental Biology
Differenziazione di cellule staminali pluripotenti umane in precursori di cellule beta pancreatiche in un sistema di coltura 2D
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Differenziazione di cellule staminali pluripotenti umane in precursori di cellule beta pancreatiche in un sistema di coltura 2D

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2
1College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

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Il presente protocollo descrive un metodo potenziato per aumentare la co-espressione dei fattori di trascrizione PDX1 e NKX6.1 nei progenitori pancreatici derivati da cellule staminali pluripotenti umane (hPSC) in monostrati planari. Ciò si ottiene reintegrando la matrice fresca, manipolando la densità cellulare e dissociando le cellule endodermiche.

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Biologia dello sviluppo numero 178
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