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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

2D培養系におけるヒト多能性幹細胞の膵臓ベータ細胞前駆体への分化

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Journal (Developmental Biology)

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Nuclear Migration in the Drosophila Oocyte

Journal (Developmental Biology)

Nuclear Migration in the Drosophila Oocyte

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2D培養系におけるヒト多能性幹細胞の膵臓ベータ細胞前駆体への分化

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021

December 16th, 2021 •

Bushra Memon^1,2, Essam M. Abdelalim^1,2

¹College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, ²Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

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本プロトコルは、平面単層におけるヒト多能性幹細胞(hPSC)に由来する膵臓前駆細胞におけるPDX1およびNKX6.1転写因子の共発現を増加させるための増強された方法を記載する。これは、新鮮なマトリックスを補充し、細胞密度を操作し、内胚葉細胞を解離することによって達成される。

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発生生物学第178号

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