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JoVE Journal Biochemistry
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Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Article DOI: 10.3791/63316-v • 05:50 min • January 20th, 2022
January 20th, 2022 •
1,2,3, 1,3, 1,2, 1,3
1Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz), 2Pós-graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná (UFPR), 3Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz)

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Summary
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The present work describes the steps for producing ready-to-use qPCR for T. cruzi DNA detection that can be pre-loaded on the reaction vessel and stored in the refrigerator for several months.

Tags

QPCR DNA Detection Trypanosoma Cruzi Pathogenic Organisms Gelification Protocol Ready-to-use QPCR Reactions Operator Error Recipe Calculations Aliquoting Visual Demonstration Melezitose Solution Trehalose Solution Glycogen Solution
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