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Journal / Developmental Biology / Isolering af rottefedtvæv Mesenkymale stamceller…
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JoVE Journal Developmental Biology
Isolering af rottefedtvæv Mesenkymale stamceller til differentiering i insulinproducerende celler
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Isolering af rottefedtvæv Mesenkymale stamceller til differentiering i insulinproducerende celler

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

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Fedtvævsafledte mesenkymale stamceller (Ad-MSC'er) kan være en potentiel kilde til MSC'er, der differentierer sig til insulinproducerende celler (IPC'er). I denne protokol giver vi detaljerede trin til isolering og karakterisering af rotte epididymal Ad-MSC'er efterfulgt af en enkel, kort protokol til generering af IPC'er fra de samme rotte-AD-MSC'er.

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Udviklingsbiologi udgave 186 Stamceller mesenkymale stamceller diabetes insulinproducerende celler fedtvæv differentiering
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