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Journal / Developmental Biology / Isolering av rotte fettvevsmesenchymale…
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JoVE Journal Developmental Biology
Isolering av rotte fettvevsmesenchymale stamceller for differensiering i insulinproduserende celler
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Isolering av rotte fettvevsmesenchymale stamceller for differensiering i insulinproduserende celler

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

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Fettvevsavledede mesenchymale stamceller (Ad-MSCer) kan være en potensiell kilde til MSCer som skiller seg ut i insulinproduserende celler (IPCer). I denne protokollen gir vi detaljerte trinn for isolering og karakterisering av rotte epididymale Ad-MSCer, etterfulgt av en enkel, kort protokoll for generering av IPCer fra samme rotte Ad-MSCer.

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Utviklingsbiologi utgave 186 stamceller mesenchymale stamceller diabetes insulinproduserende celler fettvev differensiering
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