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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Article DOI: 10.3791/63352-v • 08:48 min • February 16th, 2022
February 16th, 2022 •
*1, *1, 1, 1, 1, 2, 1,3
1Department of Anatomy & Cell Biology, Rush University Medical Center, 2Department of Microbial Pathogens and Immunity, Rush University Medical Center, 3Department of Pathology, Rush University Medical Center
* These authors contributed equally

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Summary
Automatic Translation

This article describes a convenient method for monitoring dynamic changes in antibody titers for two immunoglobulin isotypes concurrently (either IgA, IgM, or IgG) resulting from an immune response to SARS-CoV-2 infection or vaccination. This 'Multianalyte Covid-19 Immune Response Panel' employs three indirect immunoassays built on coded microspheres that are read using a flow-based multiplex reader with 'dual-channel' capability.

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Dynamic Monitoring Seroconversion Multianalyte Immunobead Assay Covid-19 High-throughput Solution Antibodies COVID-19 Infections Vaccination Routine Analyses Epitopes Biological Sciences Cell Signaling Studies Immune Responses Magnetic Microspheres Bead ID Lot Information Vortex Sonicate Low Protein Binding Microcentrifuge Tube Magnetic Separator Supernatant Wash Protocol
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