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Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
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Cancer Research
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JoVE Journal Cancer Research
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

DOI:
10.3791/63374-v

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08:01 min

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April 21, 2022

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, ,
1Department of Translational Molecular Pathology,University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Introduction
  • 00:45Digestion of Tumor Tissue
  • 03:16Generation of Primary Tumor Cell Line
  • 04:47Characterization and Banking of Established Primary Tumor Cell Line
  • 06:13Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this method paper is to demonstrate a robust and reproducible methodology for the enrichment, generation, and expansion of primary tumor cell lines from surgically resected pleural mesothelioma.

Tags

Keywords: Primary Malignant Pleural MesotheliomaTumor Cell ExpansionIn Vitro ModelTumor Digesting Enzymatic CocktailTumor DissociationCell StrainerTumor Digestion MediumCell CultureFibroblast Contamination

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