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Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Article DOI: 10.3791/63401-v • 13:04 min • January 18th, 2022
January 18th, 2022 •
1, 1, 2, 3, 1, 1
1Centre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, 2Optics 11 life, 3Laboratory of Biosensors and Bioelectronics, ETH Zürich

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The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.

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Soft Matter Nanoindentation Experimental Workflow Data Analysis Hydrogels Cells Stiffness Measurement Open-source Software Atomic Force Microscopy Mechanical Properties Healthy Samples Diseased Samples Nanoindentation For Soft Materials Instrument Calibration Probe Calibration Petri Dish Temperature Matching Liquid Calibration Pre-wetting The Probe
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