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JoVE Journal Bioengineering
In vivo Beeldvorming van biologische weefsels met gecombineerde twee-fotonenfluorescentie en gestimuleerde Raman-verstrooiingsmicroscopie
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In vivo Beeldvorming van biologische weefsels met gecombineerde twee-fotonenfluorescentie en gestimuleerde Raman-verstrooiingsmicroscopie

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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Gestimuleerde Raman-verstrooiing (SRS) microscopie maakt labelvrije beeldvorming van biomoleculen mogelijk op basis van hun intrinsieke vibratie van specifieke chemische bindingen. In dit protocol wordt de instrumentele opstelling van een geïntegreerde SRS- en twee-fotonenfluorescentiemicroscoop beschreven om cellulaire structuren in het ruggenmerg van levende muizen te visualiseren.

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Bio-engineering Nummer 178
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