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Journal / Bioengineering / In vivo Imagerie de tissus biologiques avec…
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JoVE Journal Bioengineering
In vivo Imagerie de tissus biologiques avec fluorescence combinée à deux photons et microscopie à diffusion Raman stimulée
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In vivo Imagerie de tissus biologiques avec fluorescence combinée à deux photons et microscopie à diffusion Raman stimulée

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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La microscopie à diffusion Raman stimulée (SRS) permet une imagerie sans étiquette des biomolécules en fonction de leur vibration intrinsèque de liaisons chimiques spécifiques. Dans ce protocole, la configuration instrumentale d’un SRS intégré et d’un microscope à fluorescence à deux photons est décrite pour visualiser les structures cellulaires dans la moelle épinière de souris vivantes.

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Bioingénierie numéro 178
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