Journal
/
Biology
/
Optisk rydding av plantevev for fluorescensavbildning
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Optical Clearing of Plant Tissues for Fluorescence Imaging

Optisk rydding av plantevev for fluorescensavbildning

DOI:
10.3791/63428-v

•

04:55 min

•

January 05, 2022

•

, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Chapters

  • 00:05Introduction
  • 00:48Fixation of Samples
  • 01:48Clearing
  • 02:21Observation
  • 02:59Results: Optical Clearing and Fluorescence Imaging of Tissues
  • 04:18Conclusion

Summary

Automatic Translation

Automatic Translation

Her er en metode beskrevet for å gjøre plantevev gjennomsiktig samtidig som stabiliteten til fluorescerende proteiner opprettholdes. Denne teknikken letter dyp avbildning av ryddet plantevev uten fysisk seksjonering.

Tags

Optical ClearingFluorescence ImagingWhole-plant ImagingPlant-microbe InteractionsFixationFluorescent ProteinVacuum InfiltrationClearing SolutionClearSeePBSSilicone FrameFluorescent Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Proper Care and Cleaning of the Microscope

Major Components of the Light Microscope

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Fluorescence Activated Cell Sorting of Plant Protoplasts

Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

Time-lapse Imaging of Mitosis After siRNA Transfection

Tandem Høytrykks Frysing og Quick Freeze Bytte av plantemateriale for transmisjonselektronmikroskopi

Visualisering Stromule Frequency med Fluorescensmikroskopi

ACT-PRESTO: biologisk vev Clearing og Immunolabeling Metoder for Volum Imaging

Read Article