Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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Chemistry

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Chemistry

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JoVE Journal Chemistry

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

DOI:

10.3791/63451-v

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08:40 min

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January 20, 2022

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Simon Ollivier², Mathieu Fanuel², Hélène Rogniaux², David Ropartz²

¹INRAE, UR BIA, F-44316 Nantes, France, ²INRAE, BIBS Facility, F-44316 Nantes, France

Chapters

00:04Introduction
01:15Tuning of the Cyclic IMS Mass Spectrometer
02:39IMS/IMS-MS Acquisition
06:08Results: Representative Results for the Cyclic IMS
07:51Conclusion

Summary

Automatic Translation

Ion mobility spectrometry (IMS) is an interesting complement to mass spectrometry for the characterization of biomolecules, notably because it is sensitive to isomerism. This protocol describes a tandem IMS (IMS/IMS) experiment, which allows the isolation of a molecule and the generation of the mobility profiles of its fragments.

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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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Chapters

Summary

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Article

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