Journal
/
Cancer Research
/
Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models
JoVE Journal
Cancer Research
Author Produced
This content is Free Access.
JoVE Journal Cancer Research
Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models

Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models

DOI:
10.3791/63457-v

•

13:43 min

•

April 01, 2022

•

, , , , , , , , , ,
1Precision Health Program,Michigan State University, 2Department of Radiology, College of Human Medicine,Michigan State University, 3Department of Pharmacology & Toxicology, College of Veterinary Medicine,Michigan State University, 4College of Osteopathic Medicine,Michigan State University, 5Advanced Materials Characterization Laboratory/Materials Research Center,Missouri University of Science and Technology, 6Institute for Quantitative (IQ) Health Science and Engineering Advanced Molecular Imaging Facility,Michigan State University, 7Department of Obstetrics Gynecology and Reproductive Biology, College of Human Medicine,Michigan State University, 8Institute for Quantitative (IQ) Health Science and Engineering,Michigan State University, 9Van Andel Research Institute, 10Miltenyi Biotec, 11Veterinary Diagnostic Laboratory, College of Veterinary Medicine,Michigan State University

Chapters

  • 00:10Introduction
  • 01:03Method Overview
  • 01:52Extended Anti-Inflammatory Treatment
  • 02:53Preoperative Preparation
  • 04:01Intraductal Injection
  • 07:20micro-CT Imaging
  • 09:32Image Analysis
  • 11:02Results
  • 11:57Conclusion

Summary

Automatic Translation

Automatic Translation

A method of intraductal injection of reagents for an ethanol-based ablative solution to the mouse mammary ductal tree for in vivo imaging and breast cancer prevention is described. Injection directly into the nipple opening allows for targeting mammary epithelial cells with minimal collateral tissue damage.

Tags

Keywords: Breast CancerIntraductal DeliveryEthanol-based Ablative SolutionX-ray VisualizationMouse ModelsMammary EpitheliaNipple OpeningTantalum Oxide NanoparticlesAnti-inflammatory TreatmentCarprofenSucralose Gel CupsMicro-CTFluoroscopy3D ReconstructionPrimary Prevention

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63457?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/63457?article_type=v

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
check_url/63457?article_type=v

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland
check_url/63457?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
check_url/63457?article_type=v

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
check_url/63457?article_type=v

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
check_url/63457?article_type=v

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
check_url/63457?article_type=v

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
check_url/63457?article_type=v

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
check_url/63457?article_type=v

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Read Article