Journal
/
Biology
/
Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma

DOI:
10.3791/63463-v

•

06:40 min

•

March 11, 2022

•

, ,
1Department of Basic Sciences,Loma Linda University, 2Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center,University of Arizona College of Medicine Tucson

Chapters

  • 00:04Introduction
  • 00:58Dissection and Isolation of Mouse Cerebral Arterioles
  • 02:30Digestion of Parenchymal Arterioles and Preparation of Endothelial Tubes
  • 03:47Utilization of Arteriolar Endothelial Tubes for the Examination of Cellular Physiology and Cellular Imaging
  • 04:54Results: Assessment of Endothelial Function
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

Intensive preparation of intact mouse cerebral endothelial "tubes" from cerebral parenchymal arterioles is illustrated for studying cerebral blood flow regulation. Further, we demonstrate the experimental strengths of this endothelial study model for fluorescence imaging and electrophysiology measurement of key cellular signaling pathways, including changes in intracellular [Ca2+] and membrane potential.

Tags

Keywords: Arteriolar EndotheliumParenchymal ArteriolesIntracerebral Blood FlowNeuronal And Glial ActivityMorphological DimensionsCalcium And Electrical SignalingEndothelial CellsEnzymatic DigestionDissociation SolutionPapainDithioerythritolCollagenaseElastaseArteriolar SegmentsEndothelial Tubes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63463?article_type=v

Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
check_url/63463?article_type=v

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
check_url/63463?article_type=v

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
check_url/63463?article_type=v

Mapping the After-effects of Theta Burst Stimulation on the Human Auditory Cortex with Functional Imaging
check_url/63463?article_type=v

Isolation and Culture of Neonatal Mouse Cardiomyocytes
check_url/63463?article_type=v

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
check_url/63463?article_type=v

Isolation and Physiological Analysis of Mouse Cardiomyocytes
check_url/63463?article_type=v

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
check_url/63463?article_type=v

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
check_url/63463?article_type=v

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Read Article