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Journal / Genetics / Preimplantation genetisk testning för aneuploidi…
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JoVE Journal Genetics
Preimplantation genetisk testning för aneuploidi på en halvledarbaserad nästa generations sekvenseringsplattform
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Preimplantation genetisk testning för aneuploidi på en halvledarbaserad nästa generations sekvenseringsplattform

Article DOI: 10.3791/63493-v • 09:30 min • August 17th, 2022
August 17th, 2022 •
*1, *2, 1, 1, 3, 4, 5, 1, 1, 1, 2, 2, 1
1Centre for Women, Children, and Reproduction, Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 3Department of Obstetrics and Gynecology, Chinese PLA General Hospital, 4Clinical Laboratory, Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
* These authors contributed equally

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Protokollet presenterar de övergripande procedurerna i labbet som krävs vid genetisk testning före implantation för aneuploidi på en halvledarbaserad nästa generations sekvenseringsplattform. Här presenterar vi de detaljerade stegen för helgenomförstärkning, DNA-fragmentval, bibliotekskonstruktion, mallberedning och sekvensering av arbetsflöde med representativa resultat.

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Genetik utgåva 186
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