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Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
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Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

DOI:
10.3791/63496-v

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09:12 min

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June 09, 2022

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, , ,
1MRC Laboratory for Molecular Cell Biology,University College London, 2NIHR Great Ormond Street Hospital Biomedical Research Centre,University College London

Chapters

  • 00:05Introduction
  • 01:08Trimming and Serial Sectioning of Embedded Samples
  • 04:12Grid Staining
  • 05:26Imaging Acquisition by TEM
  • 06:52Segmentation and 3D Reconstruction
  • 07:31Results: Segmentation and 3D Reconstruction Reviewing the Morphology of Endoplasmic Reticulum and the Associated ER-Mitochondria Contacts
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

A simple and comprehensive protocol to acquire three-dimensional details of membrane contact sites between organelles in hepatocytes from the liver or cells in other tissues.

Tags

Keywords: 3D ReconstructionInterorganelle Contact SitesHepatocytesSerial Section Electron MicroscopyOrganelle MorphologyCellular TraffickingUltra-thin SectioningMicrotomeDiamond KnifeCutting WindowRibbon SectioningSlot Grid

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