A simple and comprehensive protocol to acquire three-dimensional details of membrane contact sites between organelles in hepatocytes from the liver or cells in other tissues.
Chun Chung, G. H., Gissen, P., Stefan, C. J., Burden, J. J. Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy. J. Vis. Exp. (184), e63496, doi:10.3791/63496 (2022).