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JoVE Journal Biochemistry
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Article DOI: 10.3791/63501-v • 06:32 min • March 9th, 2022
March 9th, 2022 •
1, 1, 1, 1,2
1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS)

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Summary
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DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.

Tags

Molecular Mechanism Histone Assembly DNA Curtain Technique Chromatin Eukaryotic DNA Dynamic Alterations Cell Cycle Phase Environmental Stimuli Genomic Integrity Epigenetic Regulation DNA Metabolic Reactions Replication Transcription Repair Histone Chaperones Nucleosomes Single-molecule Imaging Technique DNA Curtain Lipid Fluidity Microfluidics Total Internal Reflection Fluorescence Microscopy (TIRFM) Protein-DNA Interactions Abo1 Schizosaccharomyces Pombe Bromodomain-containing AAA+ ATPase
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