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Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

DOI:
10.3791/63506-v

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09:43 min

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June 08, 2022

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,
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Chapters

  • 00:05Introduction
  • 00:27Proteinuria Test
  • 01:17Isolation of the Kidney Cells
  • 04:51Immunofluorescent Staining
  • 07:39Results: Characterizing Lupus Nephritis Based on Cell Infiltration and Protein Deposition in the Kidneys
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a method to track lupus progression in mice. Two additional procedures are presented to characterize lupus nephritis based on cell infiltration and protein deposition in the kidneys.

Tags

Keywords: ProteinuriaRenal InfiltrationLeukocytesRenal DepositionProteinsLupus-prone MRL/lpr MiceChronic InflammationKidneysLupusUrine CollectionKidney DissectionCell IsolationPercoll GradientLeukocyte PurificationFlow Cytometry

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