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Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
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Chapters
- 00:04Introduction
- 00:49Electrolytically Sharpened Tungsten Needles
- 01:45Preparing Larvae for Surgery
- 03:08Eye-Removal and Postoperative Care
- 04:27Dissecting Larvae to Reveal Brains
- 06:03Mounting and Imaging
- 07:01Results: Vertebrate Visual System Development in Zebrafish Larvae
- 08:17Conclusion
The article explains how to surgically remove eyes from living zebrafish larvae as the first step toward investigating how retinal input influences optic tectum growth and development. In addition, the article provides information about larval anesthetization, fixation, and brain dissection, followed by immunohistochemistry and confocal imaging.
