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Bioengineering

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JoVE Journal Bioengineering
Engenharia de Agentes Antivirais via Ressonância de Plasmon de Superfície
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Engenharia de Agentes Antivirais via Ressonância de Plasmon de Superfície

Article DOI: 10.3791/63541-v • 13:00 min • June 14th, 2022
June 14th, 2022 •
1,2
1Department of Environmental Health Sciences, University of California, Los Angeles, 2Department of General Surgery, Medical University of Vienna

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O presente protocolo descreve novas ferramentas para ensaios de ligação ao SPR para examinar a ligação de CV-N ao AH, glicoproteína S, glicanos do tipo híbrido relacionados e oligossacarídeos com alto teor de manose. O SPR é usado para determinar o KD para ligar o CV-N dimérico ou monomérico a esses glicanos.

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Bioengenharia Edição 184 Cianovirina-N ressonância plasmônica de superfície diferença de transferência de saturação-RMN picos de envelope glicano glicoproteína recombinante
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