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Journal / Biochemistry / Fingerprinting Cardiolipin in Leukocytes by Mass…
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Biochemistry

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JoVE Journal Biochemistry
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
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Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Article DOI: 10.3791/63552-v • 06:48 min • March 23rd, 2022
March 23rd, 2022 •
*1, *2, 2, 2
1Medical School, Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro
* These authors contributed equally

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Summary
Automatic Translation

This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.

Tags

Fingerprinting Cardiolipin Leukocytes Mass Spectrometry Rapid Diagnosis Barth Syndrome MALDI-TOF Diagnostic Lipid Species Clinical Laboratories Blood Samples Orbital Shaker Dextran RBCs Sedimentation Centrifuge Leukocytes Resuspension Potassium Chloride Osmotic Shock
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