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Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
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Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo

Article DOI: 10.3791/63586-v • 09:18 min • April 21st, 2022
April 21st, 2022 •
1,2, 1, 1,2
1Department of Chemistry & Biochemistry, University of Maryland, 2Department of Anatomy & Cell Biology, The George Washington University

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Summary
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Here we describe a mass spectrometry-based proteomic characterization of cell lineages with known tissue fates in the vertebrate Xenopus laevis embryo.

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Cell-lineage Guided Mass Spectrometry Proteomics Developing Embryo Spatial Resolution Temporal Resolution Vertebrate Embryo Whole Embryo Measurements Proteins Metabolites Transcripts Embryonic Cells Differentiation Molecular Mechanisms Tissue Induction Cell Fate Commitment Hair Loop Microneedle Xenopus Laevis Tissue Fate Maps Precursor Cell Vitelline Membrane Single Cells Manual Dissection Forceps Injection Needle Lineage Tracer Solution Micro Pipette Holder Multi-axis Micro Manipulator Micro Injector
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