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Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
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JoVE Journal Chemistry
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo

DOI:
10.3791/63586-v

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09:18 min

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April 21, 2022

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, ,
1Department of Chemistry & Biochemistry,University of Maryland, 2Department of Anatomy & Cell Biology,The George Washington University

Chapters

  • 00:04Introduction
  • 00:52Identification and Isolation of Single Cells
  • 01:27Labeling Cells of Interest
  • 02:42Isolation of Labeled Clones
  • 03:47Proteomic Analysis
  • 05:39Separation by Capillary Electrophoresis
  • 06:37Separation by Liquid Chromatography
  • 07:12Ionization and Detection of Peptides
  • 07:30Results: Characterization of Protein Expression in Single Cells and Cell Lineages of Xenopus laevis Embryos
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a mass spectrometry-based proteomic characterization of cell lineages with known tissue fates in the vertebrate Xenopus laevis embryo.

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