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A Heterotopic Mouse Model for Studying Laryngeal Transplantation

Article DOI: 10.3791/63619-v • 14:15 min • January 13th, 2023
January 13th, 2023 •
*1, *1,2, 1, 3,4, 1,5
1Head and Neck Regenerative Medicine Laboratory, Center for Regenerative Medicine, Mayo Clinic Arizona, 2Department of Otorhinolaryngology, University of Health Sciences, Sisli Hamidiye Etfal Training and Research Hospital, 3Department of Otolaryngology, Vanderbilt University, 4Cleveland Clinic Head and Neck Institute, 5Division of Laryngology, Department of Otolaryngology Head and Neck Surgery, Mayo Clinic Arizona
* These authors contributed equally

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The aim of this manuscript is to describe the microsurgical steps required to perform a heterotopic laryngeal transplant in mice. The advantages of this mouse model compared to other animal models of laryngeal transplantation are its cost-effectiveness and the availability of immunologic assays and data.

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Laryngeal Transplantation Heterotopic Mouse Model Donor Carotid Arteries Anastomosis Superior Thyroid Arteries Immunologic Studies Low Cost Surgical Steps Anesthesia Shaving Area Isoflurane Face Mask Depth Of Anesthesia Toe Pinch Chest And Neck Shaving Hair Removal Cream Surgical Area Povidone-iodine Alcohol Horizontal Incision Suprasternal Notch Fine Scissors Trapezoid Shaped Skin Segment Bilateral Salivary Glands
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