This article presents the integration of a spectral-focusing module and a dual-output pulse laser, enabling rapid hyperspectral imaging of gold nanoparticles and cancer cells. This work aims to demonstrate the details of multimodal nonlinear optical techniques on a standard laser scanning microscope.
Wang, C., Mansfield, J. C., Stone, N., Moger, J. Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy. J. Vis. Exp. (183), e63637, doi:10.3791/63637 (2022).