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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
DOI:

07:13 min

May 16, 2022

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Chapters

  • 00:04Introduction
  • 00:34Operating on the Laser Scanning Microscope
  • 01:30Mounting a Sample in the Microscope Stage
  • 02:05Changing the Raman Shift and Collecting a Hyperspectral Data Stack
  • 05:01Results: Hyperspectral Imaging of Gold Nanoparticles and Cancer Cells
  • 06:16Conclusion

Summary

Automatic Translation

This article presents the integration of a spectral-focusing module and a dual-output pulse laser, enabling rapid hyperspectral imaging of gold nanoparticles and cancer cells. This work aims to demonstrate the details of multimodal nonlinear optical techniques on a standard laser scanning microscope.

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