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Laser Microdissection for Species-Agnostic Single-Tissue Applications
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Laser Microdissection for Species-Agnostic Single-Tissue Applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

DOI:
10.3791/63666-v

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08:57 min

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March 31, 2022

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, , , ,
1Center for Developmental Genetics,New York University, 2Sacred Heart University, 3Baylor School

Chapters

  • 00:05Introduction
  • 01:00Removing Mothers and Larvae and Collecting Newly Hatched L1s
  • 02:00Collecting L4 Males and Hermaphrodites and Fixation
  • 03:07Laser Microdissection and Setting up the Microscope
  • 04:27Cutting
  • 06:27Results: Appearance of Caenorhabditis elegans L3 Hermaphrodites and Males, the Focal Plane for the PEN Membrane Slide for Laser Dissection, and Power Analysis Using PowsimR
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.

Tags

Keywords: Laser MicrodissectionCEL-Seq2Transcriptomic DataGene ExpressionC. ElegansNon-model SpeciesM9 BufferL1 LarvaMale And Hermaphrodite WormsTail TipsCentrifugationMethanol Wash

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