Name: Back Mechanical Sensitivity Assessment in the Rat for Mechanistic Investigation of Chronic Back Pain (Video) | JoVE Journal
Uploaded: 2022-08-30T13:00:00.000+00:00
Duration: 5 min 10 s
Description: Learn the BMS test for assessing back mechanical sensitivity in rat models of chronic back pain. This standardized Von Frey-based method enables longitudinal evaluation of therapeutic interventions with reproducible results.

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This work was supported by a grant from the Fondation Chiropratique du Qu&#233;bec and the Natural Sciences and Engineering Research Council of Canada (MP: grant #06659). The contribution of HK was supported by the Universit&#233; du Qu&#233;bec &#224; Trois-Rivi&#232;res (PAIR program). The contribution of BP was supported by the Fonds de recherche du Qu&#233;bec en Sant&#233; (FRQS) and the Fondation Chiropratique du Qu&#233;bec. The contribution of TP was supported by the Natural Sciences and Engineering Research Council of Canada. The contribution of NE and EK was supported by the Fondation Chiropratique du Qu&#233;bec. The contribution of MP was supported by the FRQS.

The experimental protocol was approved by the animal care committee of Universit&#233; du Qu&#233;bec &#224; Trois-Rivi&#232;res and conformed to the Guidelines of the Canadian Council on Animal Care and the Guidelines of the Committee for Research and Ethical Issues of the International Association for the Study of Pain (IASP). The present study used six male Wistar rats (body weight: 320-450 g; age: 18-22 weeks). The animals were obtained from a commercial source (see Table of Materials). Data from these rats are from the larger sample of a previous study30.
1. Experimental preparation
<ol>
	<li>House the animals in a temperature-controlled room in standard animal facilities with access to food and water ad libitum and a light-dark cycle of 14 h-10 h. Ensure that all animals are in good health on the day of the experiments.</li>
	<li>Generate the chronic back pain animal model following the steps below.
	<ol>
		<li>To induce chronic back pain, perform an intramuscular injection of Complete Freund Adjuvant (CFA) into the back muscles following the previous reports14,30,31.</li>
		<li>Anesthetize the animal using isoflurane (4% for induction and 2%-2.5% for maintenance).</li>
		<li>Using a 27 G needle, inject 150 &#181;L of a ready-to-use water-in-oil emulsion of CFA (see Table of Materials) into the paraspinal muscles unilaterally or bilaterally, depending on the protocol needs.</li>
		<li>Keep the injection needle in place for at least 3 min after completing the injection. For animals in the control group, use the same procedures30, but inject a solution of sterile physiological saline solution (150 &#181;L, 0.9%) instead of CFA.</li>
	</ol>
	</li>
	<li>Fabricate the test cage.
	<ol>
		<li>Make a test cage for two animals that comprises one chamber for each animal. 
		NOTE: For the present study, each chamber has the following dimensions: length x width x height: 50 x 20 x 7 cm (see Table of Materials).</li>
		<li>Mount the two contiguous chambers on four 33 cm long Plexiglass legs. Use transparent Plexiglass for the walls of the chambers, but use black Plexiglass to separate chambers to prevent animals from seeing each other.</li>
		<li>Use stainless-steel mesh made of 1 mm wire with an 8 mm inter-wire distance to make the floor and ceiling of the test cage (Figure 1).</li>
	</ol>
	</li>
</ol>
2. Back Mechanical Sensitivity (BMS) test
<ol>
	<li>Familiarize the animal with the test cage 30 min/day for 5-7 consecutive days prior to the first test. Repeat the test as needed.</li>
	<li>Anesthetize the animals using 2% isoflurane31 (see Table of Materials).</li>
	<li>In a prone position under isoflurane anesthesia, shave the back hair of the area of interest (from T6 to L6 vertebral levels) using an animal hair trimmer (see Table of Materials). For repeated measures, shave the back hair every 3 days on a day without behavioral assessment to ensure that stimuli are always applied directly to the skin. Draw a black mark on the skin with a permanent marker to ensure that filaments are always applied to the same area when repeating the test on different days.</li>
	<li>On the testing day, put the animals in the test cage for 15-30 min prior to the test until the animal is calm.</li>
	<li>During the test, apply Von Frey filaments (0.07, 0.16, 0.4, 0.6, 1, 2, 4, 6, 10, 15, and 26 g) perpendicularly to the back, always beginning with the 2 g filament and using the up-down method15 (see Table of Materials). Approach the back of the animal slowly with the filament from behind the animal.
	<ol>
		<li>Apply the filament only when the animal is awake, standing on its four paws, and not moving. Apply the filament for 2 s bilaterally to the area of interest, 10 mm from the spinous process (Figure 2), every 15-30 s. 
		NOTE: A response is considered positive if the animal exhibits one or more of the following behaviors during or immediately after the filament is applied: (1) muscle twitching, (2) arching (back extension), (3) rotation of the neck to look at the back, (4) scratching or licking the back, and (5) escaping.</li>
	</ol>
	</li>
	<li>As described previously15, if no response is observed with the application of a filament, apply the next filament with a higher force in the series. If a response is observed, use the next filament with a lower force in the series. Continue this procedure until four readings are obtained after the first behavioral change (response after a series of &#34;no response&#34; or no response after a series of &#34;response&#34;).</li>
	<li>Once data collection is completed, calculate the value representing 50% of the mechanical threshold, as described by Chaplan et al.15, using this formula: 
	50%&#160;threshold (g) = 10(Xf+k&#948;)/10 000 
	&#8203;NOTE: In this formula, &#34;Xf&#34;&#160;is the Handle Mark of the last von Frey filament that was used. &#34;k&#34; is the tabular value based on the animal&#39;s response pattern15, and &#34;&#948;&#34; is the mean of Handle Mark&#39;s increments between Von Frey filaments. Depending on the experimental design and experimental needs, only one side of the spine may be assessed to report one threshold, or two sides may be evaluated, and thresholds are reported separately or as a mean. Refer to Supplementary Table 1 for the calculation template32.</li>
</ol>
3. Animal recovery
<ol>
	<li>After the intramuscular injection is completed, discontinue anesthesia and place the animal alone in a standard housing cage for recovery.</li>
	<li>During the recovery period, examine the animal&#39;s behavior and do not leave it unattended.</li>
	<li>Confirm that the animal recovers from anesthesia and moves normally within 5 min. Then, return the animal to its usual housing cage with the other animals. 
	NOTE: At the end of the experiment, the animal is perfused through the heart with a 10% formalin solution, under deep isoflurane anesthesia (5%). The back muscles in the injected area are then extracted for histology and confirmation of inflammatory changes.</li>
</ol>

<ol><li>Hartvigsen, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((What+low+back+pain+is+and+why+we+need+to+pay+attention%5BTitle%5D)+AND+%22Lancet%22%5BJournal%5D)">What low back pain is and why we need to pay attention.</a> Lancet. 391 (10137), 2356-2367 (2018).</li>
<li>Manchikanti, L., Singh, V., Falco, F. J., Benyamin, R. M., Hirsch, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Epidemiology+of+low+back+pain+in+adults%5BTitle%5D)+AND+%22Neuromodulation%22%5BJournal%5D)">Epidemiology of low back pain in adults.</a> Neuromodulation. 17, Suppl 2 3-10 (2014).</li>
<li>Urits, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Low+back+pain%2C+a+comprehensive+review%3A+Pathophysiology%2C+diagnosis%2C+and+treatment%5BTitle%5D)+AND+%22Current+Pain+and+Headache+Reports%22%5BJournal%5D)">Low back pain, a comprehensive review: Pathophysiology, diagnosis, and treatment.</a> Current Pain and Headache Reports. 23 (3), 23(2019).</li>
<li>James, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Global%2C+regional%2C+and+national+incidence%2C+prevalence%2C+and+years+lived+with+disability+for+354+diseases+and+injuries+for+195+countries+and+territories%2C+1990-2017%3A+a+systematic+analysis+for+the+Global+Burden+of+Disease+Study+2017%5BTitle%5D)+AND+%22Lancet%22%5BJournal%5D)">Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017.</a> Lancet. 392 (10159), 1789-1858 (2018).</li>
<li>Hoy, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+systematic+review+of+the+global+prevalence+of+low+back+pain%5BTitle%5D)+AND+%22Arthritis+%26+Rheumatology%22%5BJournal%5D)">A systematic review of the global prevalence of low back pain.</a> Arthritis & Rheumatology. 64 (6), 2028-2037 (2012).</li>
<li>Shi, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+models+for+studying+the+etiology+and+treatment+of+low+back+pain%5BTitle%5D)+AND+%22Journal+of+Orthopaedic+Research%22%5BJournal%5D)">Animal models for studying the etiology and treatment of low back pain.</a> Journal of Orthopaedic Research. 36 (5), 1305-1312 (2018).</li>
<li>Olmarker, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Puncture+of+a+lumbar+intervertebral+disc+induces+changes+in+spontaneous+pain+behavior%3A+An+experimental+study+in+rats%5BTitle%5D)+AND+%22Spine%22%5BJournal%5D)">Puncture of a lumbar intervertebral disc induces changes in spontaneous pain behavior: An experimental study in rats.</a> Spine. 33 (8), 850-855 (2008).</li>
<li>Deuis, J. R., Dvorakova, L. S., Vetter, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Methods+used+to+evaluate+pain+behaviors+in+rodents%5BTitle%5D)+AND+%22Frontiers+in+Molecular+Neuroscience%22%5BJournal%5D)">Methods used to evaluate pain behaviors in rodents.</a> Frontiers in Molecular Neuroscience. 10, 284(2017).</li>
<li>Kawakami, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pathomechanism+of+pain-related+behavior+produced+by+allografts+of+intervertebral+disc+in+the+rat%5BTitle%5D)+AND+%22Spine%22%5BJournal%5D)">Pathomechanism of pain-related behavior produced by allografts of intervertebral disc in the rat.</a> Spine. 21 (18), 2101-2107 (1996).</li>
<li>Hu, S. -J., Xing, J. -L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+experimental+model+for+chronic+compression+of+dorsal+root+ganglion+produced+by+intervertebral+foramen+stenosis+in+the+rat%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">An experimental model for chronic compression of dorsal root ganglion produced by intervertebral foramen stenosis in the rat.</a> Pain. 77 (1), 15-23 (1998).</li>
<li>Xie, W. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robust+increase+of+cutaneous+sensitivity%2C+cytokine+production+and+sympathetic+sprouting+in+rats+with+localized+inflammatory+irritation+of+the+spinal+ganglia%5BTitle%5D)+AND+%22Neuroscience%22%5BJournal%5D)">Robust increase of cutaneous sensitivity, cytokine production and sympathetic sprouting in rats with localized inflammatory irritation of the spinal ganglia.</a> Neuroscience. 142 (3), 809-822 (2006).</li>
<li>Arthritis and Rheumatism. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+a+new+animal+model+for+evaluation+and+treatment+of+back+pain+due+to+lumbar+facet+joint+osteoarthritis%5BTitle%5D)+AND+%22Arthritis+and+Rheumatism%22%5BJournal%5D)">Characterization of a new animal model for evaluation and treatment of back pain due to lumbar facet joint osteoarthritis.</a> Arthritis and Rheumatism. 63 (10), 2966-2973 (2011).</li>
<li>Kobayashi, Y., Sekiguchi, M., Konno, S. -I., Kikuchi, S. -I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Increased+intramuscular+pressure+in+lumbar+paraspinal+muscles+and+low+back+pain%3A+Model+development+and+expression+of+substance+P+in+the+dorsal+root+ganglion%5BTitle%5D)+AND+%22Spine%22%5BJournal%5D)">Increased intramuscular pressure in lumbar paraspinal muscles and low back pain: Model development and expression of substance P in the dorsal root ganglion.</a> Spine. 35 (15), 1423-1428 (2010).</li>
<li>Touj, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sympathetic+regulation+and+anterior+cingulate+cortex+volume+are+altered+in+a+rat+model+of+chronic+back+pain%5BTitle%5D)+AND+%22Neuroscience%22%5BJournal%5D)">Sympathetic regulation and anterior cingulate cortex volume are altered in a rat model of chronic back pain.</a> Neuroscience. 352, 9-18 (2017).</li>
<li>Chaplan, S. R., Bach, F. W., Pogrel, J. W., Chung, J. M., Yaksh, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+assessment+of+tactile+allodynia+in+the+rat+paw%5BTitle%5D)+AND+%22Journal+of+Neuroscience+Methods%22%5BJournal%5D)">Quantitative assessment of tactile allodynia in the rat paw.</a> Journal of Neuroscience Methods. 53 (1), 55-63 (1994).</li>
<li>Deuis, J. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analgesic+effects+of+clinically+used+compounds+in+novel+mouse+models+of+polyneuropathy+induced+by+oxaliplatin+and+cisplatin%5BTitle%5D)+AND+%22Neuro-Oncology%22%5BJournal%5D)">Analgesic effects of clinically used compounds in novel mouse models of polyneuropathy induced by oxaliplatin and cisplatin.</a> Neuro-Oncology. 16 (10), 1324-1332 (2014).</li>
<li>Randall, L. O., Selitto, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+method+for+measurement+of+analgesic+activity+on+inflamed+tissue%5BTitle%5D)+AND+%22Archives+Internationales+de+Pharmacodynamie+et+de+Therapie%22%5BJournal%5D)">A method for measurement of analgesic activity on inflamed tissue.</a> Archives Internationales de Pharmacodynamie et de Therapie. 111 (4), 409-419 (1957).</li>
<li>D'Amour, F. E., Smith, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+method+for+determining+loss+of+pain+sensation%5BTitle%5D)+AND+%22Journal+of+Pharmacology+and+Experimental+Therapeutics%22%5BJournal%5D)">A method for determining loss of pain sensation.</a> Journal of Pharmacology and Experimental Therapeutics. 72 (1), 74-79 (1941).</li>
<li>Woolfe, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+evaluation+of+the+analgesic+actions+of+pethidine+hydrochlodide+%28Demerol%29%5BTitle%5D)+AND+%22Journal+of+Pharmacology+and+Experimental+Therapeutics%22%5BJournal%5D)">The evaluation of the analgesic actions of pethidine hydrochlodide (Demerol).</a> Journal of Pharmacology and Experimental Therapeutics. 80 (3), 300-307 (1944).</li>
<li>Hargreaves, K., Dubner, R., Brown, F., Flores, C., Joris, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+new+and+sensitive+method+for+measuring+thermal+nociception+in+cutaneous+hyperalgesia%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia.</a> Pain. 32 (1), 77-88 (1988).</li>
<li>Deuis, J. R., Vetter, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+thermal+probe+test%3A+A+novel+behavioral+assay+to+quantify+thermal+paw+withdrawal+thresholds+in+mice%5BTitle%5D)+AND+%22Temperature%22%5BJournal%5D)">The thermal probe test: A novel behavioral assay to quantify thermal paw withdrawal thresholds in mice.</a> Temperature. 3 (2), 199-207 (2016).</li>
<li>Allchorne, A. J., Broom, D. C., Woolf, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Detection+of+cold+pain%2C+cold+allodynia+and+cold+hyperalgesia+in+freely+behaving+rats%5BTitle%5D)+AND+%22Molecular+Pain%22%5BJournal%5D)">Detection of cold pain, cold allodynia and cold hyperalgesia in freely behaving rats.</a> Molecular Pain. 1, 36(2005).</li>
<li>Carlton, S. M., Lekan, H. A., Kim, S. H., Chung, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Behavioral+manifestations+of+an+experimental+model+for+peripheral+neuropathy+produced+by+spinal+nerve+ligation+in+the+primate%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Behavioral manifestations of an experimental model for peripheral neuropathy produced by spinal nerve ligation in the primate.</a> Pain. 56 (2), 155-166 (1994).</li>
<li>Brenner, D. S., Golden, J. P., Gereau, R. W. I. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+behavioral+assay+for+measuring+cold+sensation+in+mice%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">A novel behavioral assay for measuring cold sensation in mice.</a> PLoS One. 7 (6), 39765(2012).</li>
<li>Moqrich, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impaired+thermosensation+in+mice+lacking+TRPV3%2C+a+heat+and+camphor+sensor+in+the+skin%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin.</a> Science. 307 (5714), 1468-1472 (2005).</li>
<li>Langford, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coding+of+facial+expressions+of+pain+in+the+laboratory+mouse%5BTitle%5D)+AND+%22Nature+Methods%22%5BJournal%5D)">Coding of facial expressions of pain in the laboratory mouse.</a> Nature Methods. 7 (6), 447-449 (2010).</li>
<li>Deacon, R. M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Burrowing+in+rodents%3A+a+sensitive+method+for+detecting+behavioral+dysfunction%5BTitle%5D)+AND+%22Nature+Protocols%22%5BJournal%5D)">Burrowing in rodents: a sensitive method for detecting behavioral dysfunction.</a> Nature Protocols. 1 (1), 118-121 (2006).</li>
<li>Griffioen, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+dynamic+weight+bearing+for+measuring+nonevoked+inflammatory+hyperalgesia+in+mice%5BTitle%5D)+AND+%22Nursing+Research%22%5BJournal%5D)">Evaluation of dynamic weight bearing for measuring nonevoked inflammatory hyperalgesia in mice.</a> Nursing Research. 64 (2), 81-87 (2015).</li>
<li>Brodkin, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+and+implementation+of+a+novel+high-throughput+behavioral+phenotyping+instrument+for+mice%5BTitle%5D)+AND+%22Journal+of+Neuroscience+Methods%22%5BJournal%5D)">Validation and implementation of a novel high-throughput behavioral phenotyping instrument for mice.</a> Journal of Neuroscience Methods. 224, 48-57 (2014).</li>
<li>Paquette, T., Eskandari, N., Leblond, H., Piché, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spinal+neurovascular+coupling+is+preserved+despite+time+dependent+alterations+of+spinal+cord+blood+flow+responses+in+a+rat+model+of+chronic+back+pain%3A+implications+for+functional+spinal+cord+imaging%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Spinal neurovascular coupling is preserved despite time dependent alterations of spinal cord blood flow responses in a rat model of chronic back pain: implications for functional spinal cord imaging.</a> Pain. , (2022).</li>
<li>Tokunaga, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Attenuation+of+widespread+hypersensitivity+to+noxious+mechanical+stimuli+by+inhibition+of+GABAergic+neurons+of+the+right+amygdala+in+a+rat+model+of+chronic+back+pain%5BTitle%5D)+AND+%22European+Journal+of+Pain%22%5BJournal%5D)">Attenuation of widespread hypersensitivity to noxious mechanical stimuli by inhibition of GABAergic neurons of the right amygdala in a rat model of chronic back pain.</a> European Journal of Pain. 26 (4), 911-928 (2022).</li>
<li>Dixon, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Efficient+analysis+of+experimental+observations%5BTitle%5D)+AND+%22Annual+Review+of+Pharmacology+and+Toxicology%22%5BJournal%5D)">Efficient analysis of experimental observations.</a> Annual Review of Pharmacology and Toxicology. 20, 441-462 (1980).</li>
</ol>

The authors declare no competing interests or relationships that may lead to any conflicts of interest.

Critical steps 
The BMS test is a simple method to assess mechanical sensitivity in the back of rats, either at one time point or repeatedly over days or weeks, when changes are expected to occur (pain models) or after pharmacological or non-pharmacological intervention. Critical issues of the method include the test cage, whose dimensions must ensure that the rat is comfortable but does not move too much. The animal&#39;s back must stay accessible through the mesh ceiling for reproducible mechanica...

Low back pain (LBP) is the leading cause of disability worldwide, which has dramatic personal, economic, and social consequences1,2,3,4. Every year, approximately 37% of the population is affected by LBP5. LBP usually resolves within a few weeks but recurs in 24%-33% of individuals, becoming chronic in 5%-10% of cases2. To understand the mechanisms and impacts of LBP as well as the effects of different therapeutic interventions, several animal models of LBP have been used, mimicking clinical conditions or some components of LBP6. These mouse and rat models can be classified in one or more of the following categories: (1) discogenic LBP7,8,, (2) radicular LBP8,9,10,11, (3) facet joint osteoarthritis12, and (4) muscle-induced LBP13,14. Since the pain cannot be measured directly in non-human species, numerous tests have been developed to quantify pain-like behaviors in these models8. These tests assess behaviors evoked by a noxious stimulus (mechanical force15,16,17, thermal stimulation18,19,20,21,22,23,24,25) or produced spontaneously26,27,28,29.
The methods using mechanical stimuli include the Von Frey test15,16 and the Randall-Selitto Test17. Methods using heat stimuli include the tail flick test18, hot plate test19, Hargreaves test20, and thermal probe test21. Methods using cold stimuli include the cold plate test22, acetone evaporation test23, and cold plantar assay24. Methods for spontaneous behaviors include the grimace scales26, burrowing27, weight-bearing and gait analysis28, as well as an automated behavioral analysis29. Despite these numerous available tests, none of them is designed specifically for back pain models.
The objective of this study is to lay down a simple and accessible test to assess mechanical sensitivity in the back of rats. The technique is largely based on the Von Frey test applied to the plantar surface of the hind paw15,16. The basic principle of the Von Frey test is to use a series of monofilaments to the region of interest, delivering constant pre-determined forces. A response is considered positive if the rat shows a nocifensive behavior. The mechanical threshold can then be calculated based on the filaments that evoked responses. In the present study, a simple and accessible method adapted from the Von Frey test is provided to determine mechanical sensitivity in the back of rats.

<table><tbody><tr><td>Aerrane (isoflurane, USP) - Veterinary Use Only</td><td>Baxter</td><td>NDC 10019-773-60</td><td>Inhalation Anaesthetic ; DIN 02225875, for inducing anasthesia</td></tr><tr><td>Complete Freund Adjuvant (CFA)</td><td>Fisher Scientific</td><td>#77140</td><td>Water-in-oil emulsion of Complete Freund Adjuvant (CFA) with killed cells of &lt;em&gt;Mycobacterium butyricum&lt;/em&gt;.</td></tr><tr><td>Male Wistar Rats</td><td>Charles River Laboratories</td><td></td><td>body weight: 320&amp;ndash;450 g; age: 18-22 weeks.</td></tr><tr><td>Penlon Sigma Delta Vaporizer</td><td>Penlon</td><td>990-VI5K-SVEEK</td><td>Penlon Sigma Delta Vaporizer used for anasthesia</td></tr><tr><td>Sharpie Permanent Marker</td><td>Sharpie</td><td>BC23636</td><td>Permanent Marker, Fine Point, Black</td></tr><tr><td>Test cage</td><td>Custom-made</td><td></td><td>Width: 20 cm;&amp;nbsp; Length: 50 cm; Height from the bottom to the top: 40 cm; Height from the bottom mesh to the top of the cage: 7 cm; Wall thickness: 5 mm; Mesh: 1 mm wire with an 8 mm inter-wire distance&amp;nbsp;&amp;nbsp;&amp;nbsp;</td></tr><tr><td>Von Frey Filaments</td><td>Aesthesio, Precise Tactile Sensory Evaluator</td><td>514000-20C</td><td>Filaments from 0.07 g to 26 g</td></tr><tr><td>Wahl Professional Animal, ARCO Cordless Pet Clipper, Trimmer Grooming&amp;nbsp;</td><td>Wahl</td><td>Kit #8786-1201</td><td>Animal hair trimmer, for shaving purposes, zero blade&amp;nbsp;</td></tr></tbody></table>

back mechanical sensitivity assessment in the rat for mechanistic investigation of chronic back pain

Back Mechanical Sensitivity Assessment in the Rat for Mechanistic Investigation of Chronic Back Pain

Low back pain is the leading cause of disability worldwide, with dramatic personal, economic, and social consequences. To develop novel therapeutics, animal models are needed to examine the mechanisms and effectiveness of novel therapies from a translational perspective. Several rodent models of back pain are used in current investigations. Surprisingly, however, no standardized behavioral test was validated to assess mechanical sensitivity in back pain models. This is critical to confirm that animals with presumed back pain present local hypersensitivity to nociceptive stimuli, and to monitor sensitivity during interventions designed to relieve back pain. The objective of this study is to lay down a simple and accessible test to assess mechanical sensitivity in the back of rats. A test cage was fabricated specifically for this method; length x width x height: 50 x 20 x 7 cm, having a stainless-steel mesh on the top. This test cage allows the application of mechanical stimuli to the back. To perform the test, the back of the animal is shaved in the region of interest, and the test area is marked to repeat the test on different days, as needed. The mechanical threshold is determined with Von Frey filaments applied to the paraspinal muscles, utilizing the up-down method described previously. The positive responses include (1) muscle twitching, (2) arching (back extension), (3) rotation of the neck (4) scratching or licking the back, and (5) escaping. This behavioral test (Back Mechanical Sensitivity (BMS) test) is useful for mechanistic research with rodent models of back pain for the development of therapeutic interventions for the prevention and management of back pain.

The method was used in a previous study, in which full data and statistics were presented to compare back mechanical sensitivity between CFA and control rats30. Representative individual data (mean of left and right thresholds) from six rats included in the previous study are presented in Figure 3 and Table 1. At baseline, mechanical sensitivity was similar between groups. Intramuscular injection of CFA in the lumbar muscles caused a marked increase i...

To develop novel therapeutic interventions for the prevention and management of back pain, animal models are required to examine the mechanisms and effectiveness of these therapies from a translational perspective. The present protocol describes the BMS test, a standardized method to assess back mechanical sensitivity in the rat.

Low back pain is the leading cause of disability worldwide, with dramatic personal, economic, and social consequences. To develop novel therapeutics, ...

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Research

JoVE Journal

Behavior

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Learn the BMS test for assessing back mechanical sensitivity in rat models of chronic back pain. This standardized Von Frey-based method enables longitudinal evaluation of therapeutic interventions with reproducible results.

Back Mechanical Sensitivity Assessment in the Rat for Mechanistic Investigation of Chronic Back Pain

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