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Journal / Developmental Biology / Estabelecendo organoides do dente humano como uma …
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Developmental Biology

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JoVE Journal Developmental Biology
Estabelecendo organoides do dente humano como uma poderosa ferramenta para pesquisa mecanicista e terapia regenerativa
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Estabelecendo organoides do dente humano como uma poderosa ferramenta para pesquisa mecanicista e terapia regenerativa

Article DOI: 10.3791/63671-v • 11:02 min • April 13th, 2022
April 13th, 2022 •
1, 2, 2, 1
1Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute, KU Leuven (University of Leuven), 2Biomedical Research Institute (BIOMED), UHasselt, Hasselt University

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Apresentamos um protocolo para desenvolver culturas organoides epiteliais a partir do dente humano. Os organoides são robustamente expansíveis e recapitulam as células-tronco epiteliais do dente, incluindo sua capacidade de diferenciação de ameloblast. O modelo organoide único fornece uma ferramenta promissora para estudar a biologia dentário humano (células-tronco) com perspectivas para abordagens regenerativas dentárias.

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Biologia do Desenvolvimento Edição 182
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