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Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
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Bioengineering
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JoVE Journal Bioengineering
Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

DOI:
10.3791/63672-v

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06:53 min

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May 04, 2022

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, , , ,
1Department of Chemical Engineering,University of Mississippi, 2Department of Biomedical Engineering,University of Mississippi

Chapters

  • 00:04Introduction
  • 00:44Flow Cell Preparation
  • 01:25Actomyosin Bundle Preparation
  • 02:30Force Measurements Using Optical Trap
  • 05:45Results: Measuring Myosin Ensemble Force Generation Using Optical Tweezers
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

Formation of actomyosin bundles in vitro and measuring myosin ensemble force generation using optical tweezers is presented and discussed.

Tags

Optical TweezersActin Filament BundlesMyosin Ensemble MechanicsCytoskeletonPLL-coated CoverslipRhodamine ActinCasein SolutionBiotinylated ActinBead SuspensionLaser Power

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