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Biology

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Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum
 

Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

Article DOI: 10.3791/63682-v 09:07 min April 6th, 2022
April 6th, 2022

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Summary

The present protocol details the preparation and visualization of fixed tissue of the Drosophila pupal notum. It can be used for either intact or wounded tissue, and the original architecture of the tissue is preserved. The procedures for dissecting, fixing, and staining are all described in this article.

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Dissecting Fixing Visualizing Drosophila Pupal Notum Immunohistochemistry Antibody Stains Notum Epithelium DNA-staining In Situ Hybridization Pupae Dissection Scope Microscope Slide Adhesive Glue Paraffin Film Centrifuge Tube Lid Microliter Pipette Tip Cover Slip
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