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CRISPR-genredigeringsverktøy for analyse av mikroRNA-klyngenettverk
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CRISPR-genredigeringsverktøy for analyse av mikroRNA-klyngenettverk

Article DOI: 10.3791/63704-v • 10:40 min • April 25th, 2022
April 25th, 2022 •
1, 1, 1, 1
1Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School

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Denne protokollen beskriver en høy gjennomstrømningsklynget regelmessig interspaced korte palindromiske repetisjoner (CRISPR) genredigeringsarbeidsflyt for mikroRNA-klyngenettverksanalyse som tillater rask generering av et panel av genetisk modifiserte cellelinjer som bærer unike miRNA-klyngemedlemsslettingskombinasjoner så store som 35 kb i et enkelt eksperiment.

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Biologi utgave 182 CRISPR induserbar Cas9 microRNA knockout miR-888 cluster
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