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JoVE Journal Chemistry
Ekstraktion af ikke-proteinaminosyrer fra cyanobakterier til væskekromatografi-tandem massespektrometrianalyse
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Ekstraktion af ikke-proteinaminosyrer fra cyanobakterier til væskekromatografi-tandem massespektrometrianalyse

Article DOI: 10.3791/63779-v • 08:14 min • December 9th, 2022
December 9th, 2022 •
1, 1, 2, 2, 1
1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

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Denne protokol beskriver ekstraktionen af ikke-proteinaminosyrer fra biologiske matricer via trichloreddikesyre (TCA) proteinudfældning og syrehydrolyse før analyse ved anvendelse af væskekromatografi-tandem massespektrometri.

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Kemi udgave 190 Aminosyre proteinudfældning trichloreddikesyre (TCA) ikke-proteinaminosyre BMAA cyanobakterier
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