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Extraktion av icke-proteinaminosyror från cyanobakterier för vätskekromatografi-tandemmasspektrometrianalys
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Extraktion av icke-proteinaminosyror från cyanobakterier för vätskekromatografi-tandemmasspektrometrianalys

Article DOI: 10.3791/63779-v • 08:14 min • December 9th, 2022
December 9th, 2022 •
1, 1, 2, 2, 1
1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

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Detta protokoll beskriver extraktionen av icke-proteinaminosyror från biologiska matriser via triklorättiksyra (TCA) proteinutfällning och syrahydrolys före analys med vätskekromatografi-tandemmasspektrometri.

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Kemi utgåva 190 aminosyra proteinutfällning triklorättiksyra (TCA) icke-proteinaminosyra BMAA cyanobakterier
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