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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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Summary
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The present protocol provides a practical solution that allows for the measurement of apoptosis, mitochondrial membrane potential, and DNA damage in human sperm with a single cytometer.

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Flow Cytometry Biomarkers Sperm Functional Defects Conventional Semen Parameters Idiopathic Infertility Sperm Apoptosis Mitochondrial Membrane Potential (MMP) DNA Damage FCM Techniques Annexin V-fluorescein Isothiocyanate (FITC)/propidium Iodide (PI) Staining 5,5& 8242;,6,6& 8242;-tetrachloro-1,1& 8242;,3,3& 8242;-tetraethyl-benzimidazolylcarbocyanine Iodide (JC-1) Probe Sperm Chromatin Structure Assay (SCSA) Acridine Orange (AO) Staining
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