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Isolamento de Preadipócitos de Embriões de Broiler Chick
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Isolamento de Preadipócitos de Embriões de Broiler Chick

Article DOI: 10.3791/63861-v • 06:32 min • August 4th, 2022
August 4th, 2022 •
1, 1, 1, 1, 1
1Department of Animal Science, University of Tennessee

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O presente protocolo descreve um método simples para isolar pré-apócitos do tecido adiposo em embriões de frango. Este método permite o isolamento com alto rendimento, cultura primária e diferenciação adipogênica de pré-apócitos. Manchas de óleo Vermelho O e mancha lipídica/DNA mediram a capacidade adipogênica de células isoladas induzidas com meios de diferenciação.

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Biologia do Desenvolvimento Edição 186
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