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Journal / Biochemistry / Purification and Quality Control of Recombinant…
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Biochemistry

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JoVE Journal Biochemistry
Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Article DOI: 10.3791/63871-v • 11:50 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 2, 2, 3, 2, 1
1Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Institut Curie, Université PSL, Sorbonne Université

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Summary
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In vitro reconstitution of cytoskeletal proteins is a vital tool to understand the basic functional properties of these proteins. The present paper describes a protocol to purify and assess the quality of recombinant septin complexes, which play a central role in cell division and migration.

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Purification Quality Control Recombinant Septin Complexes Cell-Free Reconstitution Protocol High-quality Septin Complexes Addgene Plasmids Sulfur Reconstitution Purification Process Protein Expression Bacterial Culture IPTG Induction Hetero-oligomers Pellet Cells Lysis Buffer Sonication
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