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Bottom-Up In Vitro Methods to assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins…
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Bottom-Up In Vitro Methods to assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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Article
DOI:
10.3791/63889-v
•
09:09 min
•
August 17th, 2022
Bottom-Up In Vitro Methods to assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
August 17th, 2022
•
Brieuc Chauvin*1, Koyomi Nakazawa*1, Alexandre Beber1,7, Aurélie Di Cicco1, Bassam Hajj1, François Iv2, Manos Mavrakis2, Gijsje H. Koenderink3, João T. Cabral4, Michaël Trichet5, Stéphanie Mangenot*6, Aurélie Bertin*1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 4Department of Chemical Engineering, Imperial College London, 5Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), 6Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, 7Institute of Biotechnology, Czech Academy of Sciences, BIOCEV
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
셉틴은 세포골격 단백질이다. 그들은 지질 막과 상호 작용하고 미크론 규모에서 막 곡률을 감지 할 수 있습니다. 우리는 막 변형, 곡률에 민감한 셉틴 결합 및 셉틴 필라멘트 초 구조를 분석하기위한 상향식 시험관 내 방법론을 설명합니다.