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Biology
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Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy
 

Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy

Article DOI: 10.3791/63904-v 09:52 min May 18th, 2022
May 18th, 2022

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Summary

Studies of cell wall biomechanics are essential for understanding plant growth and morphogenesis. The following protocol is proposed to investigate thin primary cell walls in the internal tissues of young plant organs using atomic force microscopy.

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Tags

Mechanical Properties Primary Cell Wall Living Plant Organs Atomic Force Microscopy Biomechanical Characterization Non-fixed Cell Walls Non-dehydrated Cell Walls Internal Tissues Vibratome Sectioning Agarose Layer Specimen Block Hexagonal Truncated Pyramid Cyanoacrylate Adhesive Vibratome Stage Section Thickness Blade Speed Vibration Frequency
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