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Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography
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JoVE Journal Medicine
Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

DOI:
10.3791/63945-v

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08:21 min

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October 11, 2022

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, , , ,
1Laboratory of Preclinical Research, Department of Research and Innovation,Groupe Hospitalier Paris Saint Joseph, 2Inserm UMR S 999, Pulmonary Hypertension: Pathophysiology and Novel Therapies, Marie Lannelongue Hospital,Groupe Hospitalier Paris Saint Joseph, 3Department of Cardiac Surgery,Marie Lannelongue Hospital

Chapters

  • 00:05Introduction
  • 01:02Heart Procurement and Instrumentation for Normothermic Ex Situ Heart Perfusion
  • 03:06Connection to the NESP Machine and Resuscitation of the Heart
  • 04:15Working Mode Procedure
  • 04:54Pressure-Volume (PV) Loop Assessment with the Conductance Method
  • 05:48Epicardial Echocardiography Assessment of the Heart in a Working State
  • 06:42Results: Resuscitation of the Heart During Normothermic Ex Situ Heart Perfusion
  • 07:39Conclusion

Summary

Automatic Translation

Automatic Translation

A reliable noninvasive approach for functional assessment of the donor heart during normothermic ex situ heart perfusion (NESP) is lacking. We herein describe a protocol for ex situ assessment of myocardial performance using the epicardial echocardiography and conductance catheter method.

Tags

Keywords: Cardiac PerformanceEx Situ PerfusionPressure-volume LoopsEchocardiographyHemodynamic AssessmentHeart TransplantationPrimary Graft FailureCardiac CannulationCardioplegiaHeart Procurement

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