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A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture

A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture

DOI:
10.3791/63962-v

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05:16 min

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March 17, 2023

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, , , ,
1Inflammation Core Facility, Institute of Biomedical Sciences,Academia Sinica

Chapters

  • 00:04Introduction
  • 00:44Transfection and AlPcS2a Staining
  • 03:00Sample Imaging and Light Illumination
  • 03:47Results: AlPcS2a-Mediated Chromophore‐Assisted Laser Inactivation for Studying patiotemporal Damage of Intracellular Vesicles in Live Cells
  • 04:25Conclusion

Summary

Automatic Translation

Automatic Translation

AlPcS2a-mediated chromophore-assisted laser inactivation (CALI) is a powerful tool for studying spatiotemporal damage of intracellular vesicles (IVs) in live cells.

Tags

Keywords: Photodynamic ApproachIntracellular Vesicle RuptureEndocytosisSignal PathwaysChromophore-assisted Laser InactivationSubcellular DamageHeLa CellsGal3-GFPAlPcS2aLysosomeConfocal Microscopy

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