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Journal / Bioengineering / Geração de Microambientes Celulares Multicue por…
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Bioengineering

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JoVE Journal Bioengineering
Geração de Microambientes Celulares Multicue por UV-Fotopatterning de Substratos de Cultura Celular Tridimensional
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Geração de Microambientes Celulares Multicue por UV-Fotopatterning de Substratos de Cultura Celular Tridimensional

Article DOI: 10.3791/63988-v • 09:30 min • June 2nd, 2022
June 2nd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Biomedical Engineering, Eindhoven University of Technology, 2Institute for Complex Molecular Systems, Eindhoven University of Technology

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Tradicionalmente, a cultura celular é realizada em substratos planares que imitam mal o ambiente natural das células in vivo. Aqui descrevemos um método para produzir substratos de cultura celular com geometrias curvas fisiologicamente relevantes e proteínas extracelulares micropatteradas, permitindo investigações sistemáticas sobre o sensoriamento celular dessas pistas extracelulares.

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Bioengenharia Edição 184 Moldagem PDMS geometria do substrato fotopatterning UV sem máscara orientação de contato curvatura microambiente celular multi-sinal revestimento de proteínas
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