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Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination
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1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences,Wuyi University
Chapters
- 00:04Introduction
- 00:51Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
- 02:54Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
- 04:24Neural Induction (Days 5 7)
- 05:25Embedding in the Basement Membrane Matrix (Days 7 10)
- 06:52Organoid Maturation (Days 10 40)
- 07:21Results: Analysis of Cerebral Organoids
- 08:41Conclusion
Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.
