Facilitating Cerebral Organoid Culture <em>via</em> Lateral Soft Light Illumination

Bangzhu Chen; Qinrong Lin; Nengqing Liu; Diyu Chen; Yinghui Zhang; Xiaofang Sun

doi:10.3791/63989

Journal

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Bioengineering

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Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Facilitating Cerebral Organoid Culture <em>via</em> Lateral Soft Light Illumination

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

2,478 Views

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09:10 min

•

June 06, 2022

DOI:

10.3791/63989-v

DOI:

10.3791/63989-v

•

09:10 min

June 06, 2022

•

2466 Views

Bangzhu Chen^1,2, Qinrong Lin^1,2, Nengqing Liu^1,2, Diyu Chen^1,2, Yinghui Zhang³, Xiaofang Sun^1,2

¹Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University ²Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes ³School of Biotechnology and Health Sciences,Wuyi University

Summary

Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.