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Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
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Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

DOI:
10.3791/63999-v

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09:00 min

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June 10, 2022

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, , ,
1Department of Kinesiology and Applied Physiology, College of Health Sciences,University of Delaware, 2Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine,University of Illinois at Chicago, 3University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute,University of Delaware

Chapters

  • 00:04Introduction
  • 00:51Tissue Dissection and Cleaning
  • 04:39Digestion of Isolated Arteries for Isolation of Endothelial Cells
  • 06:01Staining of Endothelial Cells Prior to Flow Cytometry
  • 07:06Results: Identification of Endothelial Cells Following Arterial Tissue Digestion
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

Tags

Keywords: Vascular Endothelial CellsVascular BedsVascular Cell BiologyVascular DysfunctionAdipose Depot IsolationHEPES BufferPeritoneal Cavity

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