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Biology

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Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
 

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Article DOI: 10.3791/63999-v
June 10th, 2022

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Summary June 10th, 2022

This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

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