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Journal / Genetics / Indfangning af kromosomkonformation på tværs af…
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Genetics

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JoVE Journal Genetics
Indfangning af kromosomkonformation på tværs af længdeskalaer
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Indfangning af kromosomkonformation på tværs af længdeskalaer

Article DOI: 10.3791/64001-v • 10:15 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1,2, 1
1Department of Systems Biology, University of Massachusetts Medical School, 2Howard Hughes Medical Institute

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Hi-C 3.0 er en forbedret Hi-C-protokol, der kombinerer formaldehyd og disuccinimidylglutarat tværbindinger med en cocktail af DpnII- og DdeI-restriktionsenzymer for at øge signal-støj-forholdet og opløsningen af kromatininteraktionsdetektion.

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Genetik udgave 191 Kromosomkonformationsfangst tværbinding begrænsningsfordøjelse kromatinsløjfer prikker topologisk associerende domæner opdeling
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