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In vitro Apikal-out enteroid model af nekrotiserende enterocolitis
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In vitro Apikal-out enteroid model af nekrotiserende enterocolitis

Article DOI: 10.3791/64003-v • 09:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

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Denne protokol beskriver en apikal-ud nekrotiserende enterocolitis (NEC)-in-a-dish model ved hjælp af tyndtarmsindatoider med omvendt polaritet, hvilket giver adgang til den apikale overflade. Vi leverer en immunfluorescerende farvningsprotokol til påvisning af NEC-relateret epitelforstyrrelse og en metode til at bestemme levedygtigheden af apikale enteroider, der udsættes for NEC-in-a-dish-protokollen.

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