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In vitro Modelo enteróide apical-out de enterocolite necrotizante
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In vitro Modelo enteróide apical-out de enterocolite necrotizante

Article DOI: 10.3791/64003-v • 09:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

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Este protocolo descreve um modelo de enterocolite necrosante a apical (NEC)-in-a-dish utilizando pequenos enteróides intestinais com polaridade invertida, permitindo o acesso à superfície apical. Fornecemos um protocolo de coloração imunofluorescente para detectar interrupção epitelial relacionada à NEC e um método para determinar a viabilidade de enteróides apical-out submetidos ao protocolo NEC-in-a-dish.

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Medicina Edição 184
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