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Immunology and Infection

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JoVE Journal Immunology and Infection
High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology
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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

Article DOI: 10.3791/64012-v • 03:42 min • May 18th, 2022
May 18th, 2022 •
1, 2, 3, 1
1Department of Otolaryngology - Head & Neck Surgery, Johns Hopkins University School of Medicine, 2W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, 3Department of Pathology, Johns Hopkins University School of Medicine

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Summary
Automatic Translation

This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.

Tags

High-Speed Temporal Bone Sectioning Assessment COVID-19 Middle Ear Pathology Protocol Time Reduction Rapid Assessment Otopathology Time-sensitive Studies Inner Ear Pathology Harvest Autopsy Cranial Nerves Bony Incision Osteotome Temporal Bone Petrous Ridge Sharp Dissection Soft Tissue Specimen Removal Submersion In Formalin Tissue Decalcification
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