Journal
/
Biology
/
Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions

Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions

DOI:
10.3791/64027-v

•

09:54 min

•

July 22, 2022

•

, ,
1Department of Biology,Emory University, 2Department of Physics,Emory University

Chapters

  • 00:05Introduction
  • 00:47Preparation and Chemical Permeabilization of Worms
  • 03:41Mechanical Disruption of Individual Worms
  • 07:28Results: Heterogeneous Bacterial Colonization of the C. elegans Intestine
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a 96-well disruption of individual bacterially colonized Caenorhabditis elegans following cold paralysis and surface bleaching to remove external bacteria. The resulting suspension is plated on agar plates to allow accurate, medium-throughput quantification of bacterial load in large numbers of individual worms.

Tags

Keywords: Caenorhabditis ElegansBacterial InteractionsSingle-worm DataMechanical Disruption96-well FormatMedium ThroughputQuantificationWorm MeasurementsM9TX01OP50SDS/DTT SolutionChemical PermeabilizationWorm Cuticle

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64027?article_type=v

Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
check_url/64027?article_type=v

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
check_url/64027?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/64027?article_type=v

Using Coculture to Detect Chemically Mediated Interspecies Interactions
check_url/64027?article_type=v

A Screenable In Vivo Assay for Mitochondrial Modulators Using Transgenic Bioluminescent Caenorhabditis elegans
check_url/64027?article_type=v

Techniques to Induce and Quantify Cellular Senescence
check_url/64027?article_type=v

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
check_url/64027?article_type=v

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice
check_url/64027?article_type=v

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
check_url/64027?article_type=v

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Read Article