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Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
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JoVE Journal Cancer Research
Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

DOI:
10.3791/64030-v

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10:13 min

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June 21, 2022

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, , ,
1Rensselaer Polytechnic Institute, 2Albany Medical College

Chapters

  • 00:04Introduction
  • 00:47Preparing Tumor Aggregates
  • 02:20Optical Coherence Tomography Imaging
  • 02:58Image Analysis
  • 05:17Spatially Refined Regional Plug Method
  • 08:12Results: Analysis of MDA‐MB‐231 and AU565 MCTS Models
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol develops an image-based technique for rapid, non-destructive, and label-free regional cell density and viability measurement within 3D tumor aggregates. Findings revealed a cell-density gradient, with higher cell densities in core regions than outer layers in developing aggregates and predominantly peripheral cell death in HER2+ aggregates treated with Trastuzumab.

Tags

Keywords: Non-destructive EvaluationTumor AggregatesDrug TreatmentCell DensityCell ViabilityTumor ModelTherapeutic ResponseAggregate ModelsDrug ScreeningCell CultureBasement Membrane Matrix3D Cell CultureOptical Coherence Tomography (OCT)

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